As you might have noticed from the previous posts, comments in Python are defined mainly by the # sign. This is the signal used for single line comment like

#this is a single line comment

If you are used to C++, this would be equivalent to //.

On the other hand, multi line comments are defined by triple double quotes """, opening and closing, similar to C++ /* ... */, like this

"""this is a multi
line comment"""

Just a note. Resuming bioinformatics mode.